Journal: The Journal of Clinical Investigation

Article Title: Claudin-2 deficiency associates with hypercalciuria in mice and human kidney stone disease

doi: 10.1172/JCI127750

Figure Lengend Snippet: (A–C) Confocal microscopy of papillary sections from Cldn2–/y mice shows von Kossa staining of deposits (pseudocolored white) colabeled with the following tubule markers: (A) Inner medullary collecting duct (AQP2) and thin descending limbs (AQP1). (B) Thin descending limbs (AQP1) and thin ascending limbs (CLC-K). (C) Inner medullary collecting ducts (AQP2) and vasa recta (MECA32). Association of calcium deposits with tubule markers is infrequent, but instances of colocalization with AQP1- or CLC-K–positive thin limbs are occasionally seen (arrows in A and B). Scale bars: 50 μm. (D and E) Electron microscopy of papillae from 5-month-old Cldn2–/y mice shows small mineral deposits within type 4 thin descending limb cells with frequent tight junctions (arrowheads) (D) as well as type 3 thin ascending limb cells lacking these features (E). Scale bars: 2 μm.

Article Snippet: The primary antibodies used were CLC-K (1:200; Alomone Labs ACL-004), AQP1 (1:500; Abcam ab9566), AQP2 (1:500; a gift from Mark Knepper, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA), MECA32 (1:100; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, Iowa, USA).

Techniques: Confocal Microscopy, Staining, Electron Microscopy